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J Islam Dent Assoc Iran 2019, 31(1): 8-13 Back to browse issues page
Effect of Arginine, Protamine, and Aqueous Extracts of Green Tea and Aloe Vera Against Enterococcus faecalis
Sanaz Najafi1, Maryam Ghasempour * 2, Abolfazl Davoodabadi3, Sohrab Kazemi4
1- Student, Research Committee, Babol University of Medical Sciences, Babol, Iran
2- Associate Professor, Oral Health Research Center, Institute of Health, Babol University of Medical Sciences, Babol, Iran , ma_ghasempour_ir @yahoo.com
3- Assistant Professor, Department of Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
4- Assistant Professor, Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sci-ences, Babol, Iran
Abstract:   (1154 Views)
Background and Aim: Because of complexity in root canals, irrigating solutions are needed in addition to mechanical instrumentation for thorough cleansing of the root canal system. This in-vitro study was designed to determine the inhibitory effect of arginine, protamine, and aqueous extracts of green tea and aloe vera against Enterococcus faecalis (E. faecalis), which causes endodontic failure.
Materials and Methods: In this experimental study, aqueous extracts of green tea and aloe vera and protamine at a concentration of 400 mg/ml and arginine at a concentra-tion of 160 mg/ml were used. E. faecalis was cultured on Mueller-Hinton broth, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the test materials against these microorganisms were determined using serial dilutions and according to the microdilution test. The positive control was 2.5% sodium hypochlorite (NaOCl).
Results: Aqueous extract of aloe vera has high antibacterial activity against E. faecalis (MIC=12.5 mg/ml and MBC=100 mg/ml), and protamine had the least effect (MIC=400 mg/ml and MBC>400 mg/ml) compared to other test materials. NaOCl, with MIC and MBC of 0.25 mg/ml against E. faecalis, showed higher antibacterial activity compared to other test materials.
Conclusion: Among the tested materials, except for NaOCl as a positive control, aqueous extract of aloe vera showed better antibacterial properties against E. faecalis.
Keywords: Arginine, Protamines, Green Tea, Aloe vera, Enterococcus faecalis, Minimum Inhibitory Concentrations, Microbial Sensitivity Tests
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Type of Study: Orginal | Subject: Endodentics
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Najafi S, Ghasempour M, Davoodabadi A, Kazemi S. Effect of Arginine, Protamine, and Aqueous Extracts of Green Tea and Aloe Vera Against Enterococcus faecalis . J Islam Dent Assoc Iran. 2019; 31 (1) :8-13
URL: http://jidai.ir/article-1-2001-en.html

Volume 31, Issue 1 (1-2019) Back to browse issues page
Journal of Islamic Dental Association of Iran


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