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:: Volume 32, Issue 1 And 2 (1-2020) ::
J Islam Dent Assoc Iran 2020, 32(1 And 2): 30-36 Back to browse issues page
Comparison of Salivary Peroxidase in HIV Positive Patients and Healthy Controls: A Case-Control Study
Fatemeh Ahmadi-Motamayel1, Samaneh Vaziri–Amjad * 2, Poorandokht Davoodi3, Mohammad Taghi Goodarzi4, Jalal Poorolajal5, Hamidreza Abdolsamadi6
1- Associate professor, Dental Implant Research Center and Dental Research Center, Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Assistant professor, Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , samanehdent83@yahoo.com
3- Assistant Professor, Department of Oral Medicine, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran
4- Professor, Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
5- Associate Professor, Research Center for Health Sciences and Department of Epidemiology & Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
6- Full Professor, Dental Implant Research Center and Dental Research Center, Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract:   (349 Views)
Background and Aim: Saliva, as an easy diagnostic tool, has various defense mecha-nisms such as immunological, enzymatic and antioxidant defense systems. Salivary peroxidase is one of the most important salivary antioxidants. Human immunodeficiency virus (HIV) positive and acquired immunodeficiency syndrome (AIDS) patients have decreased antioxidant and increased oxidative stress levels. Therefore, the aim of this study was to compare the level of salivary peroxidase in HIV positive patients and healthy controls.
Materials and Methods: In this case-control study, 49 HIV positive patients and 49 healthy controls were selected as the case and control group, respectively. Demographic, clinical, and paraclinical information was recorded in a checklist. Next, 5 mL of unstimulated whole saliva was collected during 5 min in sterile Falcon tubes. Peroxidase activity of the saliva was measured by the Gelycol method. Data were ana-lyzed by SPSS 11 via t-test and Chi-square test.
Results: The salivary peroxidase level was slightly higher in the case group in comparison with the healthy control group, but this difference was not statistically significant (P=0.157). Dental status of the case group was significantly different from the control group (P=0.029). The mean age of the case group was also significantly higher than the control group (P=0.005). In the case group, females had a higher frequency than males.
Conclusion: The results showed slightly, but not significantly, higher level of peroxidase in the study group than the healthy controls. Minimal change in salivary peroxidase in HIV positive patients may be related to the early stage of their disease and good function of the immune system.
Keywords: Saliva, Peroxidases, HIV, Antioxidants
Full-Text [PDF 583 kb]   (105 Downloads)    
Type of Study: Orginal | Subject: Oral Medicine
References
1. Awodele O, Olayemi SO, Nwite JA, Adeyemo TA. Investigation of the levels of oxidative stress parameters in HIV and HIV-TB co-infected patients. J Infect Dev Ctries. 2012 Jan 12;6(1): 79-85.
2. Yousaf MZ, Zia S, Babar ME, Ashfaq UA. The epi-demic of HIV/AIDS in developing countries; the current scenario in Pakistan. Virol J. 2011 Aug 12;8:401.
3. Abdolsamadi HR, Goodarzi MT, Mortazavi H, Robati M, Ahmadi-Motemayel F. Comparison of salivary antioxidants in healthy smoking and non-smoking men. Chang Gung Med J. 2011 Nov-Dec; 34(6):607-11.
4. Bartosz G. Total antioxidant capacity. Adv Clin Chem. 2003;37:219-92.
5. Ramirez V, González A, De la Rosa E, González M, Rivera I, Hernández C, et al. Oral lesions in Mexican HIV-infected patients. J Oral Pathol Med. 1990 Nov;19(10):482-5.
6. Dalmadi L. The role of saliva in the oral defense mechanisms. Fogors SV. 2004 Oct;97 (5):199-203.
7. Nagler RM, Klein I, Zarzhevsky N, Drigues N, Reznick AZ. Characterization of the differentiated antioxidant profile of human saliva. Free Radic Biol Med. 2002 Feb 1;32(3): 268-77.
8. Samaranyake L. saliva as a diagnostic fluid. Int Dent J. 2007 Oct;57(5):295-9.
9. Puy CL. The role of saliva in maintaining oral health and as an aid to diagnosis. Med Oral Patol Oral Cir Bucal. 2006 Sep; 11(5):E449-55.
10. Akalin FA, Baltacioglu E, Alver A, Karabulut E. Lipid peroxidation levels and total oxidant status in serum, saliva and gingival crevicular fluid in patients with chronic periodontitis. J Clin Perio-dontol. 2007 Jul;34(7):558-65.
11. Baltacioglu E, Akalin FA, Alver A, Balaban F, Un-sal M, Karabulut E. Total antioxidant capacity and superoxide dismutase activity levels in serum and gingival crevicular fluid in post-menopausal women with chronic periodontitis. J Clin Periodontol. 2006 Jun;33 (6):385-92.
12. Panjamurthy K, Manoharan S, Ramachandran CR. Lipid peroxidation and antioxidant status in patients with periodontitis. Cell Mol Biol Lett. 2005 Feb;10(2):255-64.
13. Reznick AZ, Klein I, Eiserich JP, Cross CE, Nagler RM. Inhibition of oral peroxidase activity by cigarette smoke: in vivo and in vitro studies. Free Radic Biol Med. 2003 Feb 1;34(3):377-84.
14. Marecotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Mi-crobiol Mol Biol. 1998 Mar;62(1):71-109.
15. Preethi B, Pyati A, Dodawad R. Evaluation of flow rate, pH, buffering capacity, calcium, total protein and total antioxidant levels of saliva in caries free and caries active children - An in vivo study. Indian J Clin Biochem. 2010 Oct;21(3): 289-94.
16. Toljanic JA, siddiqui AA, patterson GL, Irwin ME. An evaluation of a dentifrice containing salivary peroxidase elements for the control of gingival disease in patients with irradiated head and neck cancer. J Prosthet Dent. 1996;76(3): 292-296.
17. Cole S, Langkamp-Henken B, Bender B, Findley K, Herrlinger-Garcia K, Uphold C. Oxidative stress and antioxidant capacity in smoking and nonsmoking men with HIV/acquired immunodeficiency syndrome. Nutr Clin Pract. 2005 Dec;20(6):662-7.
18. Kanehira T, Shibata K, Kashiwazaki H, Inoue N, Morita M. Comparison of antioxidant enzymes in saliva of elderly smokers and non-smokers. Gerodontology. 2006 Mar;23(1): 38-42.
19. Romero-Alvira D, Roche E. The keys of oxidative stress in acquired immune deficiency syndrome apoptosis. Med Hypotheses. 1998 Aug;51(2):169-173.
20. Coaccioli S, Crapa G, Fantera M, Del Giorno R, Lavagna A, Standoli ML, et al. Oxidant/antioxidant status in patients with chronic HIV infection. Clin Ter. 2010;161(1):55-8.
21. Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993 Sep 20;694:72-7.
22. Pruitt K, Kamau D, Miller K, Mansson-Rahemtulla B, Rahmetulla F. Quantitative, standardized assays for determin-ing the concentrations of bovine lactoperoxidase, human salivary peroxidase and human myeloperoxidase. Anal Biochem. 1990 Dec; 191 (2):278-286.
23. Müller F, Svardal A, Nordoy I, Berge R, Aukrust P, Frøland S. Virological and immunological effects of antioxidant treatment in patients with HIV infection. Eur J Clin Invest. 2000 Oct;30(10):905-14.
24. Klein I, Nagler RM, Toffler R, van Der Vliet A, Reznick AZ. Effect of cigarette smoke on oral peroxidase activity in human saliva: role of hydrogen cyanide. Free Radic Biol Med. 2003 Dec;35(11):1448-52.
25. Ihalin R, Loimaranta V, Tenovuo J. Origin, struc-ture, and biological activities of peroxidases in human saliva. Arch Biochem Biophys. 2006 Jan 15;445(2):261-8.
26. Reznick AZ, Shehadeh N, Shafir Y, Nagler RM. Free radicals related effects and antioxidants in saliva and serum of adolescents with Type 1 diabetes mellitus. Arch Oral Biol. 2006 Aug;51(8):640-8.
27. Wei PF, Ho KY, Ho YP, Wu YM, Yang YH, Tsai CC. The investigation of glutathione peroxidase, lac-toferrin, myeloperoxidase and interleukin-1beta in gingival crevicular fluid: implications for oxi-dative stress in human periodontal diseases. J Periodontal Res. 2004 Oct;39(5):287-93.
28. Bentur L, Mansour Y, Brik R, Eizenberg Y, Nagler RM. Salivary oxidative stress in children during acute asthmatic attack and during remission. Respir Med. 2006 Jul;100(7):1195-201.
29. Canakci C, Cicek Y, Yildirim A, Sezer U, Canakci V. Increased levels of 8 hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients. Eur J Dent. 2009 Nov;3(2):100-6.
30. Guentsch A, Preshaw P, Bremer-Streck S, Klinger G, Glockmann E, BW S. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment. Clin Oral Investig. 2008 Dec;12(4):345-52.
31. Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga P. Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis, and acquired immunodeficiency syndrome. Free Radic Biol Med. 2002 Jul 15;33(2):192-200.
32. Davoodi P, Hamian M, Nourbaksh R, Motamayel FA. Oral Manifestations Related To CD4 Lymphocyte Count in HIV-Positive Pa-tients. J Dent Res Dent Clin Dent Pros. 2010 Au-tumn; 4(4):115-119.
33. Hegde AM, Rai K, Padmanabhan V. Total antioxidant capacity of saliva and its relation with early childhood caries and rampant caries. J Clin Pediatr Dent. 2009 Spring;33(3):231-4.
34. Tulunoglu O, Demirtas S, Tulunoglu I. Total an-tioxidant levels of saliva in children related to caries, age, and gender. Int J Paediatr Dent. 2006 May;16(3):186-91.
35. Vučićević-Boras V, Topić B, Cekić-Arambašin A, Stavljenić-Rukavina A, Zadro R, Devčić T. Meas-urement of salivary peroxidase values in un-stimulated and stimulated whole saliva in a den-tal student population. Acta Stomatol Croat. 2001;35(3):357-9.
36. Lemos SS, Oliveira FA, Vencio EF. Periodontal disease and oral hygiene benefits in HIV seropositive and AIDS patients. Med Oral Patol Oral Cir Bucal. 2010 Mar 1;15(2):e417-21.
37. Lagendran S, Archunan G, Rengarajan RL, Mu-thuKumar S, Ilayaraja R, Amarnat A, et al. Char-acterization of antioxidant profile in human sa-liva during menstrual cycle. Int J Biol Med Res. 2011;2(1):382-9.
38. Atkinson JC, Baum BJ. Salivary enhancement: Current status and future therapies. J Dent Edu. 2001 Oct;65(10):1096-101.
39. kaufman E, lamster Ib. Analysis of saliva for per-iodontal diagnosis. J Periodontol. 2000 July; 27(7):453-65.
40. Garg N, Singh R, Dixit J, Jain A, Tewari V. Level of lipid peroxides and antioxidants in smoker and non smoker. J Periodontal Res. 2006 Oct;41(5):405-10.
41. Al-Rawi NH. Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. Diab Vasc Dis Res. 2011 Jan;8(1):22-8.
42. Serafini M. The role of antioxidants in disease prevention. Medicine. 2006 Oct;34(12): 533-5.
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Ahmadi-Motamayel F, Vaziri–Amjad S, Davoodi P, Goodarzi M T, Poorolajal J, Abdolsamadi H. Comparison of Salivary Peroxidase in HIV Positive Patients and Healthy Controls: A Case-Control Study. J Islam Dent Assoc Iran. 2020; 32 (1 and 2) :30-36
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Volume 32, Issue 1 And 2 (1-2020) Back to browse issues page
Journal of Islamic Dental Association of Iran

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