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:: Volume 26, Issue 4 (10-2014) ::
J Islam Dent Assoc Iran 2014, 26(4): 239-244 Back to browse issues page
Synergistic Antimicrobial Efficacy of 2% Chlorhexidine and 3% Hydrogen Peroxide for Infected Root Canals of Extracted Human Teeth: An Ex-Vivo Study
Safora Sahebi1, Hossein Mirhadi *2, Mohammad Moatamedifar3, Ali Ariamanesh4
1- Associate Professor, Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences. Shiraz, Iran
2- Assistant Professor, Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences. Shiraz, Iran , mirhadid@sums.ac.ir
3- Professor, Department of Microbiology, School of Medicine, Shiraz University of Medical Sciences. Shiraz, Iran
4- Endodontist
Abstract:   (3596 Views)

Background and Aim: An endodontic irrigation solution with low toxicity and high an-timicrobial activity is required in the process of endodontic treatment. Using a combina-tion of intracanal irrigants is one solution to achieve this goal. The aim of this study was to evaluate the synergistic antimicrobial efficacy of chlorhexidine and hydrogen peroxide against bacteria in the infected extracted human root canals.

Materials and Methods: Forty-two teeth with periapical lesions were chosen for this study. The teeth were extracted and preserved in normal saline at 37°C for less than 24 hours. Canals were prepared with nickel titanium rotary files (S1 to F3) and irrigated with 10cc of the respective irrigants (group 1:14 teeth irrigated with 3% hydrogen peroxide group 2: 14 teeth irrigated with 2% chlorhexidine and group 3: 14 teeth irrigated with a combination of 3% hydrogen peroxide and 2% chlorhexidine). Samples were obtained with paper cones before and after root canal preparation (S1, S2) and transferred to a mi-crobiology lab for colony counting. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests.

Results: The results showed that all the understudy irrigants significantly decreased the bacterial colony count. A combination of chlorhexidine and hydrogen peroxide was sig-nificantly more effective than hydrogen peroxide.

Conclusion: Chlorhexidine is an effective irrigant with high antimicrobial activity but its antimicrobial efficacy does not significantly increase in combination with hydrogen per-oxide.

Keywords: Chlorhexidine, Hydrogen peroxide, Synergistic effect, Root canal irrigant, Antibacterial effect
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Type of Study: Orginal | Subject: Endodentics
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Sahebi S, Mirhadi H, Moatamedifar M, Ariamanesh A. Synergistic Antimicrobial Efficacy of 2% Chlorhexidine and 3% Hydrogen Peroxide for Infected Root Canals of Extracted Human Teeth: An Ex-Vivo Study. J Islam Dent Assoc Iran. 2014; 26 (4) :239-244
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Volume 26, Issue 4 (10-2014) Back to browse issues page
Journal of Islamic Dental Association of Iran

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